Serum beta 2 microglobulin and lactate dehydrogenase 2 isoenzyme as markers of bone marrow infiltration in non-Hodgkin lymphoma

Objective: To determine the levels of serum lactate dehydrogenase 2 [LD2] Isoenzyme and beta 2 microglobulin [beta 2m] in patients of non-Hodgkin's lymphoma [NHL] and to correlate these levels in NHL patients with and without bone marrow infiltration

Study Design: Cross sectional study

Place and Duration of Study: Post Graduate Medical Institute, Lahore and Centre of Excellence in Molecular Biology [CEMB], Lahore, from 2008 to 2010

Material and Methods: The study was conducted on 80 subjects irrespective of age and sex and divided into three groups i.e. group A comprising 20 normal healthy controls, group B 30 patients of NHL without bone marrow infiltration while group C consisted of 30 NHL patients with bone marrow infiltration. Serum beta 2m and LD2 isoenzyme levels were determined in the subjects and the values were compared with healthy age and sex matched controls. The estimations were made prior to the institution of chemotherapy

Results: Serum beta 2 microglobulin and LD2 levels were significantly raised in NHL patients compared with controls. There was also significant difference when the values were compared between the patients of NHL with and without bone marrow infiltration

Conclusion: The levels of beta 2m and LD2 showed positive correlation with the extent of the disease, so we conclude that non-invasive parameters are useful indicator of the extent of the disease

Correlation of patterns of bone marrow infiltration and biochemical factors in non-hodgkin lymphoma

Objectives: To investigate the patterns of bone marrow involvement in in Non-Hodgkin's Lymphoma [NHL] patients and to correlate the patterns with beta2 microglobulin [beta2m] and lactate dehydrogenase 2 [LD2] levels in these patients

Methods: It was a cross sectional study which was conducted in two years at Post Graduate Medical Institute, Lahore and at Centre of Excellence in Molecular Biology [CEMB], Lahore. The study was conducted on 50 subjects irrespective of age and sex divided into two groups i.e. Group-A comprising 20 normal healthy controls while Group-B consisted of 30 patients of NHL with bone marrow infiltration. Bilateral bone marrow trephine biopsy was done to assess the patterns of bone marrow infiltration. Serum beta2m and LD2 isoenzyme levels were determined in already diagnosed 30 patients of NHL. The values were compared with 20 healthy age and sex matched controls. Correlation coefficients were determined using Pearson's Correlation Coefficient. The estimations were made prior to the institution of chemotherapy

Results: beta2m and LD2 levels were significantly [p-Value < 0.05] raised in NHL patients with disease advancement and were compared with controls. These serological markers showed negative correlation [-0.235 for beta2m and -0.133 for LD2] with the spread of disease and patterns of involvement in NHL patients

Conclusion: By observing the patterns of bone marrow involvement in NHL patients possible guidelines about prognosis and treatment protocols can be obtained as the serological markers levels correlate well with the spread of disease and patterns of involvement in NHL patients

Serum lactate dehydrogenase 2 isoenzyme as marker of bone marrow infiltration in non hodgkin lymphoma

To assess the level of serum lactate dehydrogenase 2 [LD2] in the patients of NHL with and without bone marrow infiltration. Lactate Dehydrogenase [LDH] is commonly increased in patients with haemopoietic malignancies and has been shown to be of prognostic value in patients with Non- Hodgkin lymphoma [NHL] especially. Serum LD2 isoenzyme level was determined in already diagnosed 60 patients of NHL. Patients were divided into two groups, 30 patients with bone marrow infiltration [group B] and remaining without infiltration [group C]. The values were compared with 20 healthy age and sex matched contrtols [groupA]. The estimations were made prior to the institution of chemotherapy. LD2 level was significantly raised in NHL patients compared with controls. There was also significant difference when the values were compared between the patients of NHL with and without bone marrow infiltration. The levels showed positive correlation with the extent of the disease. We conclude that the above mentioned non invasive parameter is useful indicator of the extent of the disease

Beta-2-microglobulin as a marker of extent of disease in non-hodgkin lymphoma

Lymphomas are the malignancies of lymphoreticular system. These malignant lymphocytes accumulate either by duplicating faster than normal or they can live longer than normal. Malignant lymphomas represent clonal malignancies in which the majority of the cells are frozen at a single stage of normal differentiation. Two broad types of lymphomas are named as Hodgkin disease and Non-Hodgkin lymphoma. Serum Beta 2 microblobulin [beta2m] is commonly increased in patients with haemopoietic malignancies and have been shown to be of prognostic value in patients especially with Non-Hodgkin lymphoma [NHL]. Serum beta2m level was determined in already diagnosed [n=60] patients of NHL. They were divided into two groups, 30 patients with bone marrow infiltration [group B] and the remaining without infiltration [group C]. The values were compared with 20 healthy age and sex matched controls [group A]. The estimations were made prior to the institution of chemotherapy. beta2m level was significantly raised in NHL patients compared with controls. There was also a significant difference when the values were compared between the patients of NHL with and without bone marrow infiltration. The levels showed positive correlation with the extent of the disease. We conclude that the above mentioned non invasive parameter is a useful indicator of the extent of the disease